20,522Result(s)
withinBiomedicine
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Effective Mapping by Sequencing to Isolate Causal Mutations in the Tomato Genome
Forward genetic analysis remains as one of the most powerful tools for assessing gene functions, although the identification of the causal mutation responsible for a given phenotype has been a tedious and time...
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Ewing Sarcoma-Specific (Re)expression Models
Gene expression and knockdown systems are powerful tools to study the function of single genes and their pathway interaction. Plasmid transfection and viral transduction have revolutionized the field of molecu...
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In Vitro Differentiation of Tumor-Associated Macrophages from Monocyte Precursors with Modified Melanoma-Conditioned Medium
Tumor-associated macrophages (TAMs) are one of most important components of the tumor microenvironment. Although many assays have been developed to differentiate monocytes into macrophages (Mϕ) for studying th...
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Predicting Hot Spots Using a Deep Neural Network Approach
Targeting protein–protein interactions is a challenge and crucial task of the drug discovery process. A good starting point for rational drug design is the identification of hot spots (HS) at protein–protein i...
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Simulating Evolution in Asexual Populations with Epistasis
I show how to use OncoSimulR, software for forward-time genetic simulations, to simulate evolution of asexual populations in the presence of epistatic interactions. This chapter emphasizes the specification of...
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Capillary Electrophoresis–Mass Spectrometry of Hydrophilic Metabolomics
Metabolomics is defined as the comprehensive identification and quantification of small molecules that provide a holistic view of cellular metabolism. The metabolomic network is downstream of the central dogma...
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Analysis of Recombination at Yeast Telomeres
Upon telomerase inactivation telomeres are getting shorter at each round of DNA replication and progressively lose capping functions and hence protection against homologous recombination. In addition, telomera...
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Characterization and Activity of TIM-1 and IL-10-Reporter Expressing Regulatory B Cells
In addition to their role in humoral immunity, B cells can exhibit regulatory activity. Such B cells have been termed regulatory B cells (Bregs). Bregs have been shown to inhibit inflammatory immune responses ...
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In Vitro 3D Models of Tunable Stiffness
Three-dimensional models of spheroid formation have been routinely used in the cancer field to test the colony forming capacity of malignant cells in an in vitro setting. Use of such a model provides a robust ...
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Glioblastoma: State of the Art of Treatments and Applications of Polymeric and Lipidic Nanomedicines
Glioblastoma multiforme (GBM) is one of the most devastating tumors affecting more than 5 in 100,000 people. Unfortunately, its diagnosis is often discovered in late stages and is normally deadly, having a lif...
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Computational Detection of Plant RNA Editing Events
Computers are able to systematically exploit RNA-seq data allowing us to efficiently detect RNA editing sites in a genome-wide scale. This chapter introduces a very flexible computational framework for detecti...
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Evaluation of Diagnostic Tests
As technology advances, diagnostic tests continue to improve and each year, we are presented with new alternatives to standard procedures. Given the plethora of diagnostic alternatives, diagnostic tests must b...
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Purification and Characterization of Murine MZ and T2-MZP Cells
The spleen is the second major reservoir of B cells in the adult. In the spleen, cells, generated in the bone marrow, are selected, mature, and become part of the peripheral B-cell pool. Murine spleen comprise...
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In Vivo Binding of Recombination Proteins to Non-DSB DNA Lesions and to Replication Forks
Homologous recombination (HR) has been extensively studied in response to DNA double-strand breaks (DSBs). In contrast, much less is known about how HR deals with DNA lesions other than DSBs (e.g., at single-s...
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Metabolomics of Small Numbers of Cells Using Chemical Isotope Labeling Combined with Nanoflow LC-MS
Performing metabolome analysis using a small number of cells is desirable and required in many areas of biological research. However, it presents a major analytical challenge to detect and quantify a large num...
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以证据为基础的决策ion-Making 2: Systematic Reviews and Meta-Analysis
The number of studies published in the biomedical literature has dramatically increased over the last few decades. This massive proliferation of literature makes clinical medicine increasingly complex, and inf...
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Detection of DNA Adduct Formation in Rat Lungs by a Micro-HPLC/MS/MS Approach
Aldehydes are abundantly present in tobacco smoke and in urban air pollution and are endogenously generated as products of the lipid peroxidation process. These molecules can react with DNA bases forming mutag...
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Immune-to-Brain Signaling Effects on the Neural Substrate forReward: Behavioral Models ofAversion,Anhedonia, andDespair
The role of immune-to-brain signaling in regulating mood and motivational states has received increasing interest, as clinical studies have uncovered a link between systemic inflammation and treatment-resistan...
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Infant Rabbit Model for Studying Shiga Toxin-ProducingEscherichia coli
Animal models represent part of the arsenal available to researchers studying the pathophysiology of potentially deadly human pathogens such as Shiga toxin-producingEscherichia coli(STEC). The optimal model may...
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Evaluating Melanoma Viability and Proliferation in 3D Microenvironments
Researchers often aim to incorporate microenvironmental variables such as the dimensionality and composition of the extracellular matrix into their cell-based assays. A technical challenge created by introduct...
